![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 54, Issue 3, 514-524, September 1998
Department of Pharmacology, Emory University School of Medicine,
and Program in Molecular Therapeutics and Toxicology, Graduate Division
of Biological and Biomedical Sciences, Emory University, Atlanta,
Georgia 30322
Expression of the angiotensin II type 1 receptor (AT1-R)
mRNA in vascular smooth muscle cells (VSMC) is down-regulated by a
variety of agonists, including growth factors, agonists of
G
q protein-coupled receptors, and activators of adenylyl
cyclase. To determine whether cAMP-dependent protein kinases (PKA)
participates in AT1-R mRNA down-regulation controlled by
multiple classes of receptors, a PKA inhibitor peptide (PKI) was
developed and expressed in rat VSMC as a fusion with the enhanced green
fluorescent protein (eGFP). PKA activity elicited both by forskolin and
angiotensin II is suppressed in cells expressing this fusion protein
(PKI-eGFP), but platelet-derived growth factor-BB does not stimulate
PKA activity in this preparation. PKI-eGFP expression fully inhibits
the forskolin-stimulated down-regulation of AT1-R mRNA
levels and blocks 50% of the effect elicited by angiotensin II. This
indicates that PKA plays a substantial role in angiotensin
II-stimulated AT1-R mRNA down-regulation. However,
inhibition of PKA has no effect on AT1-R mRNA
down-regulation caused by platelet-derived growth factor-BB. These
findings show how agonists such as angiotensin II that are not normally
considered as activators of PKA can use PKA-dependent processes to
modulate gene expression. These findings also provide definitive
evidence that PKA-dependent pathways are involved in modulation of
AT1-R mRNA levels in VSMC.
This article has been cited by other articles:
|
|
 |
|
  J. Leemhuis, S. Boutillier, G. Schmidt, and D. K. Meyer The Protein Kinase A Inhibitor H89 Acts on Cell Morphology by Inhibiting Rho Kinase J. Pharmacol. Exp. Ther., March 1, 2002; 300(3): 1000 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Nickenig and D. G. Harrison The AT1-Type Angiotensin Receptor in Oxidative Stress and Atherogenesis: Part II: AT1 Receptor Regulation Circulation, January 29, 2002; 105(4): 530 - 536. [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Robida, K. Xu, M. L. Ellington, and T. J. Murphy Cyclosporin A Selectively Inhibits Mitogen-Induced Cyclooxygenase-2 Gene Transcription in Vascular Smooth Muscle Cells Mol. Pharmacol., October 1, 2000; 58(4): 701 - 708. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. de Gasparo, K. J. Catt, T. Inagami, J. W. Wright, and Th. Unger International Union of Pharmacology. XXIII. The Angiotensin II Receptors Pharmacol. Rev., September 1, 2000; 52(3): 415 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Schaffhauser, Z. Cai, F. Hubalek, T. A. Macek, J. Pohl, T. J. Murphy, and P. J. Conn cAMP-Dependent Protein Kinase Inhibits mGluR2 Coupling to G-Proteins by Direct Receptor Phosphorylation J. Neurosci., August 1, 2000; 20(15): 5663 - 5670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Howe and K. D. McCarthy Retroviral Inhibition of cAMP-Dependent Protein Kinase Inhibits Myelination But Not Schwann Cell Mitosis Stimulated by Interaction with Neurons J. Neurosci., May 15, 2000; 20(10): 3513 - 3521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Xu and T. J. Murphy Reconstitution of Angiotensin Receptor mRNA Down-regulation in Vascular Smooth Muscle. POST-TRANSCRIPTIONAL CONTROL BY PROTEIN KINASE A BUT NOT MITOGENIC SIGNALING DIRECTED BY THE 5'-UNTRANSLATED REGION J. Biol. Chem., March 10, 2000; 275(11): 7604 - 7611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Banke, D. Bowie, H.-K. Lee, R. L. Huganir, A. Schousboe, and S. F. Traynelis Control of GluR1 AMPA Receptor Function by cAMP-Dependent Protein Kinase J. Neurosci., January 1, 2000; 20(1): 89 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Adams, T. S. Obertone, X. Wang, and T. J. Murphy Relationship between Internalization and mRNA Decay in Down-Regulation of Recombinant Type 1 Angiotensin II Receptor (AT1) Expression in Smooth Muscle Cells Mol. Pharmacol., June 1, 1999; 55(6): 1028 - 1036. [Abstract] [Full Text]
|
|
|
|
|
|
K. Xu, A. M. Robida, and T. J. Murphy Immediate-early MEK-1-dependent Stabilization of Rat Smooth Muscle Cell Cyclooxygenase-2 mRNA by Galpha q-coupled Receptor Signaling J. Biol. Chem., July 21, 2000; 275(30): 23012 - 23019. [Abstract] [Full Text] [PDF]
|
|
 |
 |
 |
|
 |
 |
 |
