![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 52, Issue 6, 1010-1018, 1997
1A- and 1B-Adrenergic
Receptor Subtypes
Department of Pharmacology and Toxicology (F.L., P.A.F., F.A.G.),
Dartmouth Medical School, Hanover, New Hampshire 03755, and Departments
of
Medicine (T.N., M.K.D.),
Cell Biology (M.K.D.), and
Sarah W. Stedman
Nutrition Center (M.K.D.), Duke University, Medical Center, Durham,
North Carolina 27710
Activation of 
1-adrenergic receptors
(1-AR) increases Na+/H+ exchange
(NHE) in proximal tubule. NHE mediates the majority of active
Na+ absorption in the proximal tubule. Three
1-AR subtypes have been detected in kidney by molecular
and binding techniques. We detected message for all three
1-AR subtypes in mouse proximal tubule cells through
reverse transcription-polymerase chain reaction and Northern analysis.
To determine the 1-AR subtypes that regulate NHE in
mouse proximal tubule cells, two strategies were used: (i) antisense
oligodeoxynucleotides (ODNs) to selectively inhibit expression of
1A-, 1B-, and 1D-AR
subtypes and (ii) subtype-selective 1-AR antagonists.
Streptolysin-O permeabilization was used to introduce
antisense and sense ODNs into cells three times over 72 hr. Western
blot analysis of membranes prepared from cells treated with
1B-AR antisense ODN demonstrated that
1B-AR protein expression was reduced by 90% at 72 hr
compared with control or sense ODN treatments. Functional regulation of
NHE by 1-ARs was determined by 1-AR
agonist changes in intracellular pH (pHi) in cells grown on
coverslips and loaded with
2
,7-bis(2-carboxyethyl)-5(6)carboxyfluorescein-acetoxymethyl ester.
Antisense ODNs for 1B-AR significantly reduced
phenylephrine (PHE)-induced maximal changes in pHi by 49%.
The PHE-induced changes in pHi observed in cells treated
with 1A-AR antisense ODNs was reduced by 42%. The
selective 1A-AR antagonist WB-4101 and the 1B-AR antagonist spiperone reduce PHE-induced
pHi increases to a comparable extent. No significant
changes in pHi were observed with cells treated with
1D-AR antisense ODNs or the 1D-AR
antagonist BMY 7378 compared with untreated cells. Combined treatment
with 1A- and 1B-AR antisense ODNs and
antagonists additively inhibits PHE-induced 
pHi by 90%.
We conclude that 1A and 1B-AR but not 1D-ARs regulate NHE in proximal tubule cells.
This article has been cited by other articles:
|
|
 |
|
  T. Kimura, P. B. Allen, A. C. Nairn, and M. J. Caplan Arrestins and Spinophilin Competitively Regulate Na+,K+-ATPase Trafficking through Association with a Large Cytoplasmic Loop of the Na+,K+-ATPase Mol. Biol. Cell, November 1, 2007; 18(11): 4508 - 4518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. K. Leong, L. E. Yang, C. S. Landon, A. A. McDonough, and K.-P. Yip Phenol injury-induced hypertension stimulates proximal tubule Na+/H+ exchanger activity Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1543 - F1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Soodvilai, V. Chatsudthipong, K. K. Evans, S. H. Wright, and W. H. Dantzler Acute regulation of OAT3-mediated estrone sulfate transport in isolated rabbit renal proximal tubules Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1021 - F1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Yang, P. K. K. Leong, S. Ye, V. M. Campese, and A. A. McDonough Responses of proximal tubule sodium transporters to acute injury-induced hypertension Am J Physiol Renal Physiol, February 1, 2003; 284(2): F313 - F322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tanoue, M. Koba, S. Miyawaki, T.-a. Koshimizu, C. Hosoda, S. Oshikawa, and G. Tsujimoto Role of the {alpha}1D-Adrenegric Receptor in the Development of Salt-Induced Hypertension Hypertension, July 1, 2002; 40(1): 101 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Salvi Protecting the Myocardium From Ischemic Injury : A Critical Role for {{alpha}}1-Adrenoreceptors? Chest, April 1, 2001; 119(4): 1242 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Liu and F. A. Gesek {alpha}1-Adrenergic receptors activate NHE1 and NHE3 through distinct signaling pathways in epithelial cells Am J Physiol Renal Physiol, March 1, 2001; 280(3): F415 - F425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Skowronski, Y. Ishikawa, and H. Ishida Enhancement by Epinephrine of Benzylpenicillin Transport in Rat Small Intestine J. Pharmacol. Exp. Ther., April 1, 2000; 293(1): 128 - 135. [Abstract] [Full Text]
|
|
|
|
|
|
A. Shuprisha, R. M. Lynch, S. H. Wright, and W. H. Dantzler PKC regulation of organic anion secretion in perfused S2 segments of rabbit proximal tubules Am J Physiol Renal Physiol, January 1, 2000; 278(1): F104 - F109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. W. MOE Acute Regulation of Proximal Tubule Apical Membrane Na/H Exchanger NHE-3: Role of Phosphorylation, Protein Trafficking, and RegulatoryFactors J. Am. Soc. Nephrol., November 1, 1999; 10(11): 2412 - 2425. [Full Text]
|
|
|
|
|
|
M. Gekle, S. Mildenberger, C. Sauvant, D. Bednarczyk, S. H. Wright, and W. H. Dantzler Inhibition of initial transport rate of basolateral organic anion carrier in renal PT by BK and phenylephrine Am J Physiol Renal Physiol, August 1, 1999; 277(2): F251 - F256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Gesek {alpha}1- and {alpha}2-Adrenoceptor Control of Sodium Transport Reverses in Developing Hypertension Hypertension, January 1, 1999; 33(1): 524 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Gonzalez-Cabrera, P. L. Iversen, M. F. Liu, M. A. Scofield, and W. B. Jeffries Selective Inhibition of alpha 1B-Adrenergic Receptor Expression and Function Using a Phosphorothioate Antisense Oligodeoxynucleotide Mol. Pharmacol., June 1, 1998; 53(6): 1034 - 1039. [Abstract] [Full Text]
|
|
 |
 |
 |
|
 |
 |
 |
